کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3393995 1221436 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of the allergen filarial tropomyosin with an invertebrate specific monoclonal antibody
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Characterization of the allergen filarial tropomyosin with an invertebrate specific monoclonal antibody
چکیده انگلیسی

Tropomyosins of invertebrates are pan-allergens responsible for wide spread allergic reactions against seafood and arthropods. As invertebrate tropomyosins are highly conserved, helminth tropomyosins are likely to show properties similar to these medically important allergens. Studies with a monoclonal antibody, NR1, raised against tropomyosin of the rodent filarial nematode Acanthocheilonema viteae revealed a B cell epitope common to helminths and marine mollusks, which does not occur in vertebrate tropomyosin. This antibody detected tropomyosin of A. viteae, other filariids, nematodes, trematodes and a cestode, and recognized as well tropomyosin of oyster, squid and octopus, but not of arthropods and vertebrates. Immunohistological analyses of A. viteae, Onchocerca volvulus and other nematodes using NR1 showed that tropomyosin is located in the fibrillar part of the body wall muscles and the uterus, and is also conspicuous in muscles of the pharynx, the vagina and other organs of the nematodes. The abundance of a pan-allergen like tropomyosin in parasitic worms and the counterintuitive, but well documented protection against allergic reactivity by some chronic helminth infections is discussed.

We show that helminths contain the allergen tropomyosin and characterize the protein using a monoclonal antibody that specifically recognizes an epitope also present in mollusks.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 116, Issue 1, October 2010, Pages 61–67
نویسندگان
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