Species B of Anopheles culicifacies (Diptera: Culicidae) is reproductively less fit than species A and C of the complex

Anopheles culicifacies, the most important malaria vector of peninsular India exist as a complex of five sibling species. The member species of the complex have various biological differences including their susceptibility to malaria parasites. The present attempt is made to study and compare the fecundity of the differentially susceptible members of the An. culicifacies complex. Gravid female mosquitoes of species A, B and C were allowed to lay their eggs individually during first and second gonotrophic cycle. The eggs were counted after hatching and categorized as ‘hatched eggs’, ‘unhatched eggs’, ‘embryonated eggs’, ‘unembryonated eggs’ and ‘non/partially melanized eggs’. The data was analyzed using Student's t test, ANOVA, Chi-square and Pearson's correlation analysis. All females that were visually categorized as ‘gravid’ did not lay eggs. Species C laid maximum number of eggs per female. The eggs laid per female mosquito of each species were found to be significantly higher during second gonotrophic cycle as compared with the first gonotrophic cycle. The eggs hatched per female in species C were found to be significantly higher than that of species A and B. The poor-vector species B mosquitoes were found to be the least fecund among the members of the species complex. The unembryonated eggs constitute the biggest proportion of the unhatched eggs in species A, B and C of the species complex.