کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3394864 1221500 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Refinement of techniques for the propagation of Leishmania donovani in hamsters
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Refinement of techniques for the propagation of Leishmania donovani in hamsters
چکیده انگلیسی

Improved animal models are urgently required for drug and vaccine development against visceral leishmaniasis. Here we report refinements to the hamster model of infection that reduce the severity of the disease as well as the number of animals required to maintain infection while improving parasite yields. A comparison between infection via the intracardiac and intraperitoneal routes showed that the less commonly used intraperitoneal route is the simpler and preferred method. The KAtex latex agglutination test for visceral leishmaniasis accurately detected Leishmania donovani antigen in hamster urine as early as 6 weeks post-inoculation. With modification, this assay could be an important tool in the evaluation of experimental drugs and vaccines.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Tropica - Volume 97, Issue 3, March 2006, Pages 364–369
نویسندگان
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