کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3395092 | 1592831 | 2015 | 6 صفحه PDF | دانلود رایگان |

• Three bacteria were isolated from the endometrium with conventional culture methods.
• Isolates are anaerobic, asporulous, asaccharolytic, indole positive, positive rods.
• Sequencing revealed only 91% similarity to cultivated organisms in GenBank.
• All data suggest a novel genus and species within the Clostridium rRNA cluster III.
Three isolates of a bacterium recovered from human endometrium using conventional culture methods were characterized biochemically and subjected to 16S rRNA gene sequencing and phylogenetic analysis. Isolates were non-motile, obligately anaerobic, non-spore forming, asaccharolytic, non-cellulolytic, indole positive, Gram positive rods. Cell wall fatty acid profiling revealed C14:0, C16:0, C18:2 ω6, 9c, C18:1 ω9c and C18:0 to be the major fatty acid composition. The DNA mol % G+C was determined to be 44.2%. 16S rRNA gene sequence analysis revealed only 91% sequence similarity with the closest cultivated bacterial isolate, Saccharofermentans acetigenes. Based on genotypic and phenotypic data, all three isolates are considered to be members of the same species and data suggest it represents a novel genus and species in the order Clostridiales with an association with Clostridium rRNA cluster III within the family Ruminococcaceae. We propose the name, Mageeibacillus indolicus gen. nov., sp. nov. The type strain is BAA-2120T and CCUG 59143T.
Journal: Anaerobe - Volume 32, April 2015, Pages 37–42