کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3395470 | 1221687 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification and analysis of the Shewanella oneidensis major oxygen-independent coproporphyrinogen III oxidase gene Identification and analysis of the Shewanella oneidensis major oxygen-independent coproporphyrinogen III oxidase gene](/preview/png/3395470.png)
Shewanella oneidenesis MR-1 is a facultative anaerobe that can use a large number of electron acceptors including metal oxides. During anaerobic respiration, S. oneidensis MR-1 synthesizes a large number of c cytochromes that give the organism its characteristic orange color. Using a modified mariner transposon, a number of S. oneidensis mutants deficient in anaerobic respiration were generated. One mutant, BG163, exhibited reduced pigmentation and was deficient in c cytochromes normally synthesized under anaerobic condition. The deficiencies in BG163 were due to insertional inactivation of hemN1, which exhibits a high degree of similarity to genes encoding anaerobic coproporphyrinogen III oxidases that are involved in heme biosynthesis. The ability of BG163 to synthesize c cytochromes under anaerobic conditions, and to grow anaerobically with different electron acceptors was restored by the introduction of hemN1 on a plasmid. Complementation of the mutant was also achieved by the addition of hemin to the growth medium. The genome sequence of S. oneidensis contains three putative anaerobic coproporphyrinogen III oxidase genes. The protein encoded by hemN1 appears to be the major enzyme that is involved in anaerobic heme synthesis of S. oneidensis. The other two putative anaerobic coproporphyrinogen III oxidase genes may play a minor role in this process.
► The Shewanella oneidensis genome encodes 3 anaerobic coproporphyrinogen III oxidases (HemN).
► HemN1 appears to be the major enzyme involved in heme biosynthesis under anaerobic conditions.
► Restoration of heme biosynthesis in the hemN1 mutant can be achieved by supplementation with exogenous hemin.
Journal: Anaerobe - Volume 17, Issue 6, December 2011, Pages 501–505