کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3395845 1221709 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Three Lactobacillus strains from healthy infant stool inhibit enterotoxigenic Escherichia coli grown in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Three Lactobacillus strains from healthy infant stool inhibit enterotoxigenic Escherichia coli grown in vitro
چکیده انگلیسی

Enterotoxigenic Escherichia coli (ETEC) are a major cause of sporadic diarrhea disease in humans, affecting mainly infants in developing countries and travelers from industrialized countries visiting tropical or subtropical areas. In this study, we screen the antagonistic activity by inoculating wells among ETEC agar cultures to assess inhibition zones created by the lactobacilli spent culture supernatant (SCS) from healthy infant stool. Only three isolates possessed antagonistic activity, acid and bile tolerance and could adhere to the cultured human intestinal C2BBel (Caco-2) cell line. Isolate identification using API 50CHL strips showed that they belonged to different Lactobacillus species, i.e., Lactobacillus acidophilus RY2, Lactobacillus salivarius MM1 and Lactobacillus paracasei En4. The SCS still had an inhibitory effect on ETEC after heating (100 °C, 15 min). The lactate dehydrogenase treatment or the pH of SCS was adjusted to neutral (pH 7.2) to reduce the SCS inhibitory effect. Antimicrobial activity was performed by incubating the lactic acid bacteria (LAB)-SCS with ETEC suspension. After 4 h of co-culture, ETEC growth was inhibited. This study suggests that L. acidophilus RY2, L. salivarius MM1 and L. paracasei En4 could be used as an effective control for ETEC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Anaerobe - Volume 14, Issue 2, April 2008, Pages 61–67
نویسندگان
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