Evaluation of human antibody responses to diphtheria toxin subunits A and B in various age groups

ABSTRACTThis study aimed to evaluate human antibody responses to diphtheria toxin subunits in various age groups. Antibodies against the intact diphtheria toxin and the diphtheria toxin subunits A and B were evaluated in 1319 individuals using a double-antigen ELISA. Although high levels of protection (83.6%, 95% CI 79.2–87.4) were found in children and adolescents, the middle-aged adult population was less protected (28.8%, 95% CI 24.3–33.6). An increase in age was associated with a decrease in the frequency of protected individuals in the 0–39-year age group (p <0.001). Anti-subunit B levels correlated well (p <0.01) with levels of antibodies against the intact toxin. In children aged ≤16 years, the intervals at which the peaks in geometric mean titres of anti-subunit B antibodies were observed were found to correlate with the ages at which booster doses are administered. Overall, males appeared to be more protected than females (OR 1.67, 95% CI 1.34–2.08, p <0.001). A small group of individuals had antibody levels of ≥0.1 IU/mL against the intact toxin, but did not have protective antibody against subunit B. Determination of anti-subunit B antibody levels should help in evaluating the effectiveness of diphtheria boosters and other aspects of diphtheria immunity.