کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3405468 | 1223387 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intérêts et les limites actuelles du MALDI-TOF en microbiologie clinique
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماری های عفونی
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چکیده انگلیسی
Until the early 2000s, in the clinical microbiology laboratories, the bacterial identification was based mainly on the biochemical analysis of phenotypic characters whose interpretation required expertise. In most cases, the results was obtained after more than 18Â h. When it was taken in default, the identification was mainly obtained by molecular biology analysis such as 16S rDNA sequences. For nearly 5Â years, using MALDI-TOF mass spectrometry machine revolutionized bacterial identification in clinical bacteriology laboratories. From bacterial colonies, the results of reliable identifications are easily obtained in less than 5Â minutes. These advantages have greatly contributed to its success. When a laboratory is equipped by this device, the bulk of the identifications are provided by this new approach. The objective of this review is to analyze the reasons of the success of MALDI-TOF in clinical bacteriology, to know its present limitations and new applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal des Anti-infectieux - Volume 14, Issue 4, November 2012, Pages 159-167
Journal: Journal des Anti-infectieux - Volume 14, Issue 4, November 2012, Pages 159-167
نویسندگان
N. Degand, R. Ruimy,