Purification and immuno-gold labeling of lily mottle virus from lily leaves

• An efficient procedure was developed for purification of LMoV from infected leaves.
• Viral purity & integrity were confirmed by OD260:280, antibodies, PCR and TEM.
• An IGL assay was successfully applied to identify a lily virus for the first time.
• The IGL is the label viz. goat anti-rabbit 15 nm colloidal gold.

Lily mottle virus (LMoV) is prevalent in Lilium species worldwide causing dwarfing, flower breaking, and reduced bulb yield. In this paper, an easy to use and efficient procedure is described for purification of LMoV from lily leaves. The resulting sample is characterized by a 260/280 nm absorbance ratio of 1.20 at a concentration of 1.27 mg/ml. The procedure results in high protein purity and particle integrity as shown by UV-spectrophotometry, polyacrylamide gel electrophoresis (PAGE), Western blotting, reverse transcriptase (RT)-PCR and transmission electron microscopy (TEM) in combination with immuno-gold labeling. This is the first time that an immuno-gold labeling (IGL) assay was performed to identify a virus of lily. Purified products can be used as a source of antigen in the preparation of antibodies against LMoV and may assist in the development of a diagnostic test for LMoV and in epidemiological surveys.