کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407050 | 1593495 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation of human influenza A viruses including the pandemic subtype H1N1/2009 by conventional multiplex PCR
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کلمات کلیدی
CDCMultiplex PCRMDCKcDNA - cDNAComplementary DNA - DNA تکمیلیEpidemiology - اپیدمیولوژی(همهگیرشناسی)Diagnosis - تشخیصWorld Health Organization - سازمان بهداشت جهانیMadin Darby canine kidney - مدین داربی کلیه کلیویCenters for Disease Control and Prevention - مراکز کنترل و پیشگیری از بیماریhemagglutination inhibition - مهار هموگلوبینhemagglutinin - هماگلوتینینplaque-forming units - واحدهای پلاک سازیpandemic - پاندمیpfu - پفوWHO - که
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
April 2009 witnessed the emergence of a novel H1N1 influenza A virus infecting the human population. Currently, pandemic and seasonal influenza viruses are co-circulating in human populations. Understanding the course of the emerging pandemic virus is important. It is still unknown how the novel virus co-circulates with or outcompetes seasonal viruses. Sustainable and detailed influenza surveillance is required throughout the world including developing countries. In the present study, a multiplex PCR using four primers was developed, which was designed to differentiate the pandemic H1N1 virus from the seasonal H1N1 and H3N2 viruses, to obtain amplicons of different sizes. Multiplex PCR analysis could clearly differentiate the three subtypes of human influenza A virus. This assay was performed using 206 clinical samples collected in 2009 in Japan. Between February and April, four samples were subtyped as seasonal H1N1 and four as seasonal H3N2. All samples collected after July were subtyped as pandemic H1N1. Currently, pandemic viruses seem to have replaced seasonal viruses almost completely in Japan. This is a highly sensitive method and its cost is low. Influenza surveillance using this assay would provide significant information on the epidemiology of both pandemic and seasonal influenza.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 168, Issues 1â2, September 2010, Pages 94-97
Journal: Journal of Virological Methods - Volume 168, Issues 1â2, September 2010, Pages 94-97
نویسندگان
Yuki Furuse, Takashi Odagiri, Takashi Okada, Irona Khandaker, Kozue Shimabukuro, Rumi Sawayama, Akira Suzuki, Hitoshi Oshitani,