کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3407276 1223623 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification
چکیده انگلیسی

Red-spotted grouper nervous necrosis virus (RGNNV) causes high mortality in marine fish larvae cultured in China. To control better an outbreak of this virus, a rapid, specific and sensitive detection method based on loop-mediated isothermal amplification (LAMP) was developed. A set of four primers, two outer and two inner, was designed from RGNNV genome RNA. The LAMP reaction mix was optimized. The method was specific as no cross-reaction was observed between white spot syndrome virus, koi herpesvirus, infectious spleen and kidney necrosis virus, mud crab reovirus, and grass carp hemorrhage virus. The sensitivity of LAMP was 100-fold higher than the nested PCR in detecting the presence of RGNNV. RGNNV was detected in the brain of Trachinotus ovatus that showed typical symptoms of NNV infection, with the standardized LAMP procedure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 163, Issue 1, January 2010, Pages 123–128
نویسندگان
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