کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3407349 1223625 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Efficient fluorescence-based imaging methods for quantitating infectivity and oncolytic efficacy of Newcastle disease virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Efficient fluorescence-based imaging methods for quantitating infectivity and oncolytic efficacy of Newcastle disease virus
چکیده انگلیسی

A high throughput quantitation protocol is desired to determine the replication of various recombinant oncolytic viruses in vitro. Plaque assay is the classic method for viral infectivity quantitation but is laborious and time consuming; moreover it does not report the oncolytic efficacy of a virus. In this paper, three new imaging methods for quantitating viral infectivity are derived and evaluated: fluorescence intensity, infection counts, and infection degree. Infection of oncolytic Newcastle disease virus in human tumor and normal cells was followed over a time course by plaque assay and the imaging methods. For the latter, brightfield and green channel images were acquired at various fixed locations in the cell culture, and later analyzed. One of the imaging methods was found to be highly correlated with viral titer; the other methods are complementary to plaque assay and provide additional information like oncolytic efficacy, syncytium formation etc. The new methods significantly reduce the time and material costs required by plaque assay, and provide an efficient system for quantitating and characterizing infectivity and efficacy of oncolytic viruses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 163, Issue 2, February 2010, Pages 390–397
نویسندگان
, , , , , ,