کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407426 | 1593499 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
N-terminally His-tagged hepatitis B core antigens: Construction, expression, purification and antigenicity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: N-terminally His-tagged hepatitis B core antigens: Construction, expression, purification and antigenicity N-terminally His-tagged hepatitis B core antigens: Construction, expression, purification and antigenicity](/preview/png/3407426.png)
چکیده انگلیسی
The core antigen of the hepatitis B virus (HBcAg) has been used widely as a diagnostic reagent for the identification of the viral infection. However, purification using the conventional sucrose density gradient ultracentrifugation is time consuming and costly. To overcome this, HBcAg particles displaying His-tag on their surface were constructed and produced in Escherichia coli. The recombinant His-tagged HBcAgs were purified using immobilized metal affinity chromatography. Transmission electron microscopy and enzyme-linked immunosorbent assay (ELISA) revealed that the displayed His-tag did not impair the formation of the core particles and the antigenicity of HBcAg.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 160, Issues 1–2, September 2009, Pages 125–131
Journal: Journal of Virological Methods - Volume 160, Issues 1–2, September 2009, Pages 125–131
نویسندگان
Wei Boon Yap, Beng Ti Tey, Michelle Y.T. Ng, Swee Tin Ong, Wen Siang Tan,