کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3407484 1223629 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reverse transcription loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus in groupers
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Reverse transcription loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus in groupers
چکیده انگلیسی

The reverse transcription loop-mediated isothermal amplification (RT-LAMP) method is a sensitive nucleic acid diagnostic method that can amplify rapidly a target template; it can be applied for the diagnosis of viral disease in grouper aquaculture. In this study, two outer and two inner primers were designed from nervous necrosis virus (NNV) coat protein gene sequence. The reaction temperature and time for the detection of NNV were optimized at 65 °C for 60 min. The detection limit of RT-LAMP is 10−6 NNV-RNA from infected groupers, and more sensitive than the one-step RT-PCR and nested RT-PCR. The combination of RNA rapid extraction and RT-LAMP, the process can be completed within 2 h. Thus, the RT-LMAP is a rapid, sensitive, specific and efficient method for detection of NNV in groupers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 159, Issue 2, August 2009, Pages 206–210
نویسندگان
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