کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407673 | 1593504 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The use of Armored RNA as a multi-purpose internal control for RT-PCR The use of Armored RNA as a multi-purpose internal control for RT-PCR](/preview/png/3407673.png)
Real time reverse transcriptase-PCR (RT-PCR) is now used commonly for the detection of viral pathogens in respiratory samples. However, due to potential inhibition of the RT-PCR or inefficient extraction, this sample type can present significant challenges to accurate patient testing. The goal of this study was to create an internal control to be multiplexed in a real time RT-PCR assay for detecting a viral target in respiratory samples. This report describes an Armored RNA (aRNA) internal control developed originally to be multiplexed in a real time RT-PCR assay for detecting SARS-associated Coronavirus, but can be incorporated into any RT-PCR assay. The internal control primers and probe target a region in the coat protein gene of the E. coli F-specific bacteriophage ms2, which is contained within the aRNA.
Journal: Journal of Virological Methods - Volume 150, Issues 1–2, June 2008, Pages 73–76