کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407691 | 1223636 | 2009 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
The non-structural protein NS-1 of porcine parvovirus (PPV) could be a useful antigen for differentiating pigs infected with PPV from those vaccinated with inactivated whole virus. NS-1 was expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native NS-1 protein as determined by Western blot assay using polyclonal antibodies from pigs vaccinated with alive PPV vaccine. A simple procedure was used to purify the fusion protein. This research lays the foundation for using the NS-1 protein for clinical diagnosis of pigs infected with PPV vs. those vaccinated with inactivated whole virus vaccine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 157, Issue 1, April 2009, Pages 93–97
Journal: Journal of Virological Methods - Volume 157, Issue 1, April 2009, Pages 93–97
نویسندگان
Ting Qi, Shang-jin Cui,