کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407726 | 1223638 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses](/preview/png/3407726.png)
چکیده انگلیسی
A semi-automated, immunomagnetic capture-reverse transcription PCR (IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 155, Issue 2, February 2009, Pages 187-192
Journal: Journal of Virological Methods - Volume 155, Issue 2, February 2009, Pages 187-192
نویسندگان
C.F. Gambley, A.D.W. Geering, J.E. Thomas,