کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407834 | 1223642 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple, sensitive, specific detection of mixed infection of multiple plant viruses using macroarray and microtube hybridization
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A simple, sensitive and specific method using a cDNA macroarray to detect multiple viruses was devised. The method is used in plants such as potato and lily, which need a reliable routine diagnosis for mixed infection. The biotinylated cRNA targets were prepared using an in vitro transcription-based system that was designed especially to eliminate nonspecific hybridizations. The macroarray hybridization was carried out using a convenient, cost-effective “microtube hybridization” (MTH) system. By this method, lily viruses including Cucumber mosaic virus, Lily symptomless virus, Lily mottle virus, and Plantago asiatica mosaic virus were detected successfully from leaves or roots of lily bulbs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 153, Issue 2, November 2008, Pages 241–244
Journal: Journal of Virological Methods - Volume 153, Issue 2, November 2008, Pages 241–244
نویسندگان
Shunpei Sugiyama, Chikara Masuta, Hiroshi Sekiguchi, Taketo Uehara, Hanako Shimura, Yukio Maruta,