کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407843 | 1223642 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using G-deleted vesicular stomatitis virus to probe the innate anti-viral response
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
A method is described for the use of a G-deleted, conditionally replicating version of vesicular stomatitis virus (VSV) to measure alterations to the innate anti-viral state of cells in vitro. By co-transfecting a gene of interest with an expression vector for VSV-G one can directly measure the replication of the virus in the transfected cells as non-transfected cells will fail to produce infectious progeny due to the lack of glycoprotein in these non-transfected cells. This allows the investigator to focus exclusively on the anti-viral state induced or inhibited in the transfected cells allowing for screening and quantitative analysis of viral or cellular genes that may modify the anti-viral state.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 153, Issue 2, November 2008, Pages 276–279
Journal: Journal of Virological Methods - Volume 153, Issue 2, November 2008, Pages 276–279
نویسندگان
Frances Lai, Natasha Kazdhan, Brian D. Lichty,