کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407958 | 1593506 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Clinical performance of an in-house real-time RT-PCR assay using a fluorogenic LUX⢠primer for quantitation of human immunodeficiency virus type-1 (HIV-1)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Clinical performance of an in-house real-time RT-PCR assay using a fluorogenic LUX⢠primer for quantitation of human immunodeficiency virus type-1 (HIV-1) Clinical performance of an in-house real-time RT-PCR assay using a fluorogenic LUX⢠primer for quantitation of human immunodeficiency virus type-1 (HIV-1)](/preview/png/3407958.png)
چکیده انگلیسی
The South African National Antiretroviral Treatment Guideline recommends the use of HIV viral load assays for routine monitoring of HIV-1 positive patients on Highly Active Antiretroviral Therapy (HAART). Approved commercial HIV-1 viral load assays are expensive for developing countries where a large number of patients are treated in the public sector. The evaluation of an in-house HIV-1 viral load assay (LUX assay) is described using 458 plasma specimens. Good specificity of the LUX assay was demonstrated using 50 seronegative plasma specimens. A group of 142 HIV-1 positive patients was used to assess the agreement between the LUX assay and the COBAS Amplicor assay. An intra class correlation (ICC) coefficient of 0.85 (CI 95%) indicated good agreement between the assays. The Bland-Altman model showed good agreement between the assays for â¼87% of the results (mean 0.03 [â1.26; 1.32], CI 95%). In a cohort of 55 patients followed-up longitudinally the LUX assay showed similar declines in viral load to the COBAS Amplicor assay in response to therapy. Viral rebound was detected in 5 patients out of 55 by both assays. Thus, the LUX assay compares well to the gold standard and represents an affordable alternative for high volume testing in resource limited settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 146, Issues 1â2, December 2007, Pages 14-21
Journal: Journal of Virological Methods - Volume 146, Issues 1â2, December 2007, Pages 14-21
نویسندگان
Natela Rekhviashvili, Wendy Stevens, Edmore Marinda, René Gonin, Gwynneth Stevens, James McIntyre, Robin Wood,