کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3407972 1593506 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid and sensitive detection of Taura syndrome virus by reverse transcription loop-mediated isothermal amplification
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Rapid and sensitive detection of Taura syndrome virus by reverse transcription loop-mediated isothermal amplification
چکیده انگلیسی

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay is a novel method of gene amplification that amplifies nucleic acid with high specificity, sensitivity and rapidity, which can be applied for disease diagnosis in shrimp aquaculture. The method is performed under isothermal conditions with a set of four specially designed primers that recognize six distinct sequences of the target. In this study, using the RT-LAMP method, a protocol for detecting Taura syndrome virus which is a causative agent of Penaeus vannamei was developed. Time and temperature conditions for detection of TSV were optimized for 60 min at 63 °C. The nucleic acids of other shrimp pathogens (yellow head virus; YHV and white spot syndrome; WSSV) were not amplified by this RT-LAMP system. The detection of TSV using RT-LAMP was 10 times more sensitive than the RT-PCR but less sensitive than nested RT-PCR. However this system was more convenient, rapid, and does not require sophisticated PCR machine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 146, Issues 1–2, December 2007, Pages 125–128
نویسندگان
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