کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3407982 | 1593506 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
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چکیده انگلیسی
A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs. The detection limit of the assay was 1 × 102 RNA copies and standard curve displayed a linear range from 1 × 102 to 1 × 109 copies and a good reproducibility. The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 146, Issues 1–2, December 2007, Pages 202–208
Journal: Journal of Virological Methods - Volume 146, Issues 1–2, December 2007, Pages 202–208
نویسندگان
Gabriella Elia, Alessandra Cavalli, Costantina Desario, Eleonora Lorusso, Maria Stella Lucente, Nicola Decaro, Vito Martella, Canio Buonavoglia,