A carboxymethyl-cellulose plaque assay for feline calicivirus

The standardization of a plaque assay for feline calicivirus in Crandell Reese feline kidney cells using carboxymethyl-cellulose as an overlay medium is described in this report. This methodology gives comparable counts as compared to the standard assay, and prevents monolayer roll over and peel off, as well as easy medium removal. Cell fixation and staining is performed in a considerably reduced period of time, compared to agarose-based methods.