کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3408011 | 1593506 | 2007 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection of human papillomavirus types 45 and 51 by type-specific polymerase chain reaction
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Human papillomavirus (HPV) types 45 and 51 are both considered as high risk types for the development of human cervical cancer. To optimize the detection of these two types in clinical samples, HPV-45 and HPV-51 specific primers were designed to amplify respectively a 141 bp and a 266 bp fragment from the L1 gene by polymerase chain reaction (PCR). The sensitivity and the specificity of these two PCR reactions were determined using varying amounts of HPV DNA containing plasmids and negative and positive controls. Overall, the sensitivity for the HPV-45 plasmid DNA is 10 fg, while for HPV-51 the sensitivity is 1 fg. This is equivalent to approximately 100 and 10 HPV genome copies per PCR reaction, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 146, Issues 1–2, December 2007, Pages 405–408
Journal: Journal of Virological Methods - Volume 146, Issues 1–2, December 2007, Pages 405–408
نویسندگان
Christine Weyn, Selma Boulenouar, Vanessa Mathys, Julie Vanhoolandt, Aurore Bernis, Véronique Fontaine,