کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3408039 1223647 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct recovery of infectious Pestivirus from a full-length RT-PCR amplicon
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Direct recovery of infectious Pestivirus from a full-length RT-PCR amplicon
چکیده انگلیسی

This study describes the use of a novel and rapid long reverse transcription (RT)-PCR for the generation of infectious full-length cDNA of pestiviruses. To produce rescued viruses, full-length RT-PCR amplicons of 12.3 kb, including a T7-promotor, were transcribed directly in vitro, and the resulting RNA transcripts were electroporated into ovine cells. Infectious virus was obtained after one cell culture passage. The rescued viruses had a phenotype similar to the parental Border Disease virus strain. Therefore, direct generation of infectious pestiviruses from full-length RT-PCR cDNA products could be a valuable instrument for virus rescue, conservation and further characterization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 149, Issue 2, May 2008, Pages 330–333
نویسندگان
, , , , , ,