کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3408111 | 1593509 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Examination of real-time PCR for HIV-1 RNA and DNA quantitation in patients infected with HIV-1 BF intersubtype recombinant variants Examination of real-time PCR for HIV-1 RNA and DNA quantitation in patients infected with HIV-1 BF intersubtype recombinant variants](/preview/png/3408111.png)
The impact of HIV-1 genetic diversity on the performance of laboratory testing is an issue that has to be monitored continuously. An “in-house” real-time PCR assay was developed by the Agence Nationale de Recherche sur le SIDA (ANRS) in France for viral load (VL) quantitation based on the amplification of the HIV-1 long terminal repeat (LTR) region. This technology has not been used in Argentina yet and considering the HIV-1 diversity in the country, a comparative analysis of this assay was undertaken versus the Versant HIV-1 RNA 3.0 Assay (b-DNA). The performance was assessed on 30 drug-naïve HIV-1 infected patients who were characterized previously by phylogenetic analysis of the pol and vpu gene. The results showed that there is a significant linear correlation between values of transformed viral load logarithms measured by both, bDNA and real-time PCR assay and that this assay can be used to quantify viral load in samples from BF-infected patients with the same accuracy and reliability as for B subtype samples. The use of “in-house” real-time PCR to measure DNA in PBMCs correlated strongly with the HIV-1 RNA levels in all specimens.
Journal: Journal of Virological Methods - Volume 140, Issues 1–2, March 2007, Pages 222–227