کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3408170 1223654 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An improved method for the recovery of recombinant paramyxovirus vaccine candidates suitable for use in human clinical trials
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
An improved method for the recovery of recombinant paramyxovirus vaccine candidates suitable for use in human clinical trials
چکیده انگلیسی

We describe a method for the generation of clinical grade, live-attenuated vaccines in Vero cells entirely from cDNA plasmids. The entire electroporation procedure can be completed in less than 15 minutes and this is a significant improvement over previous lipid or electroporation based transfection techniques that also involve a heat-shock step. Importantly, the virus preparations can be generated with a minimal use of animal product derived materials, an important consideration for a vaccine candidate that is to be tested in humans. Since it is likely that all live-attenuated parainfluenza virus and pneumovirus vaccines in the future will be generated using reverse genetics, this simplified method provides guidance on how this can be achieved.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 141, Issue 1, April 2007, Pages 30–33
نویسندگان
, , , ,