کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3408281 | 1223658 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genotype differentiating RT-PCR and sandwich ELISA: Handy tools in epidemiological investigation of foot and mouth disease
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Years of molecular epidemiological surveillance has revealed co-circulation of two antigenically divergent genotypes of foot and mouth disease virus serotype A in India. Genotype differentiating RT-PCR and sandwich ELISA were developed as fast, cost-effective and user-friendly alternatives to 1D region based phylogeny for detection and differentiation of genotype VI and VII. The RT-PCR assay targeting 1D region was found to be more sensitive and authentic in distinguishing genotypes than sandwich ELISA. These assays promise to be reliable tools in the epidemiological investigation of foot and mouth disease in the country.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 143, Issue 1, July 2007, Pages 117–121
Journal: Journal of Virological Methods - Volume 143, Issue 1, July 2007, Pages 117–121
نویسندگان
Jajati Keshari Mohapatra, Saravanan Subramaniam, Chakradhar Tosh, Divakar Hemadri, Aniket Sanyal, Tamilselvan Ramasamy Periyasamy, Thaha Jamal Rasool,