کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3408526 1593511 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of real-time and conventional RT-PCR assays for the detection of potato yellow vein virus (PYVV)
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Development of real-time and conventional RT-PCR assays for the detection of potato yellow vein virus (PYVV)
چکیده انگلیسی

Potato yellow vein virus (PYVV) is considered a quarantine pathogen in the European and Mediterranean Plant Protection Organization (EPPO) area. This virus is widespread and damaging at its centre of origin in South America. Current detection methods are either time-consuming or difficult to interpret. This paper reports the development of a sensitive, high throughput, real-time reverse transcription (RT)-PCR assay, based on TaqMan® chemistry, suitable for PYVV detection. In addition, a reliable conventional RT-PCR assay for PYVV detection is also presented. Although less sensitive (1000 times less sensitive in direct comparison), this method requires less sophisticated equipment and as such should be a useful alternative to the real-time technique in some testing laboratories. The two assays presented here could assist in the implementation of quarantine measures for PYVV identification and in routine indexing of PYVV for the production of virus-free seed potatoes in areas of South America where the virus is highly damaging.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 136, Issues 1–2, September 2006, Pages 24–29
نویسندگان
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