کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3408624 1223672 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improving the sensitivity of single-strand conformation polymorphism (SSCP) to study the variability of PLMVd
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Improving the sensitivity of single-strand conformation polymorphism (SSCP) to study the variability of PLMVd
چکیده انگلیسی
Single-strand conformation polymorphism (SSCP) was used to characterize viroids. Eight cDNA clones, which showed identical profiles in preliminary existing SSCP analysis but had different sequences, were chosen to develop a sensitive SSCP technique for identifying the variability of Peach latent mosaic viroid (PLMVd). Polyacrylamide Gel Electrophoresis (PAGE) conditions were optimized to improve the sensitivity of the existing SSCP, and a modified SSCP protocol was developed. The results indicated that the modified SSCP protocol provided an overall sensitivity in identifying the variability of these clones, and showed higher resolution than the existing one and its improved versions. As shown by sequence analyses of cDNA clones of PLMVd and the modified SSCP profiles, there is no close correlation between the number of base changes and variation of the modified SSCP band patterns. The potential use of the modified SSCP analysis is discussed as a tool for viroids characterization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 135, Issue 2, August 2006, Pages 276-280
نویسندگان
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