کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3408655 1593513 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple wax-embedding method for isolation of aphid hemolymph for detection of luteoviruses in the hemocoel
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
A simple wax-embedding method for isolation of aphid hemolymph for detection of luteoviruses in the hemocoel
چکیده انگلیسی

A protocol for isolating hemolymph from viruliferous aphids has been developed. This method uses warm melted wax to immobilize the aphid. Following removal of a hind leg, the hemolymph can be collected readily. Flushing with RNase-free water allows for collection of sufficient hemolymph for RNA extraction from individual aphids. The extracted RNA was successfully used for detection of barley yellow dwarf virus (BYDV) and pea enation mosaic virus (PEMV) from individual viruliferous Rhopalosiphum padi and Acyrthosiphon pisum aphids, respectively. A TaqMan real-time RT-PCR protocol for quantitation of PEMV in the hemolymph of individual aphids was developed. The wax-embedding hemolymph collection technique provides a useful tool for studying molecular interactions between persistent and circulative plant viruses and their insect vectors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Virological Methods - Volume 132, Issues 1–2, March 2006, Pages 174–180
نویسندگان
, , ,