کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3416280 | 1593690 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Helicobacter pylori CagI is associated with the stability of CagA
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Helicobacter pylori CagI is associated with the stability of CagA Helicobacter pylori CagI is associated with the stability of CagA](/preview/png/3416280.png)
چکیده انگلیسی
• We constructed an isogenic mutant of cagI via allelic exchange.
• We performed immunoprecipitation, MALDI-TOF and pull-down to verify the interation between CagA and CagI.
• We carried out qRT-PCR and Western blotting to show that CagA was unstable in the absence of CagI.
The type Ⅳ secretion system (T4SS) of H. pylori encoded by cag pathogenicity island mediates the injection of the toxin CagA, which is considered as a paradigm for bacterial carcinogenesis. T4SS is generally composed of a set of proteins, the majority of which still remain unclear. In this study, we have identified CagI, one of the T4SS proteins, which interacted with CagA and played an important role for CagA stability.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 99, October 2016, Pages 130–134
Journal: Microbial Pathogenesis - Volume 99, October 2016, Pages 130–134
نویسندگان
Hua Wang, Yizheng Yao, Bin Ni, Yixin Shen, Xiaochun Wang, Hongxing Shen, Shihe Shao,