کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416410 1593699 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HIV-1 Tat regulates the expression of the dcw operon and stimulates the proliferation of bacteria
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
HIV-1 Tat regulates the expression of the dcw operon and stimulates the proliferation of bacteria
چکیده انگلیسی


• Endogenous HIV-1 Tat stimulates bacterial growth.
• Exogenous Tat1-86 and Tat1-101 stimulates bacterial growth.
• Genes related to cell division are regulated by HIV-1 Tat.

Infections of pathogenic bacteria are very common in acquired immunodeficiency syndrome (AIDS) patients. However, the biological effects of HIV-1 Tat on bacteria are incompletely understood. In this study, HIV-1 Tat was expressed in Escherichia coli and Pseudomonas aeruginosa (PA01) to investigate its biological effects on bacteria. Bacterial cells expressing either HIV-1 Tat1-86 (Tat1-86) or HIV-1 Tat1-72 (Tat1-72) grow significantly faster than those with either only an empty vector or an unrelated control (GFP or Rluc). Supplementation of purified HIV-1 Tat1-86 or Tat1-101 protein into bacterial culture medium stimulated the growth of both E. coli and PA01. The expression profile of certain cell division-associated genes, such as those in the division cell wall (dcw) operon (ftsA, ftsQ, ftsW and ftsZ), yafO and zipA, was altered in HIV-1 Tat1-86 expressing E. coli BL21(DE3). Furthermore, the expression of firefly luciferase (Fluc) reporter gene, when engineered for control by the dcw promoter and terminator, was enhanced by HIV-1 Tat in E. coli, confirming that HIV-1 Tat transcriptionally regulates the expression of the dcw operon. The finding that HIV-1 Tat stimulates bacterial growth whether it is produced intracellularly or applied extracellularly may have relevance for HIV patients who are highly susceptible to opportunistic bacterial infections.Contents category: Viruses –Retroviruses.The GenBank accession number for the sequence of HIV-1 Tat1-86 is AF324439.1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 90, January 2016, Pages 34–40
نویسندگان
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