کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416439 1593700 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Entamoeba invadens: Identification of a SERCA protein and effect of SERCA inhibitors on encystation
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Entamoeba invadens: Identification of a SERCA protein and effect of SERCA inhibitors on encystation
چکیده انگلیسی


• Entamoeba invadens has a calcium pump of the SERCA type (EiSERCA).
• In trophozoites EiSERCA co-localizes with calreticulin in a vesicular network distributed in all the cytoplasm.
• In cyst, vesicles containing EiSERCA are translocated close to plasma membrane.
• Thapsigargin and Cyclopiazonic acid, specific inhibitors of SERCA, reduced the number of cysts and altered their structure.

Calcium has an important role on signaling of different cellular processes, including growth and differentiation. Signaling by calcium also has an essential function in pathogenesis and differentiation of the protozoan parasites Entamoeba histolytica and Entamoeba invadens. However, the proteins of these parasites that regulate the cytoplasmic concentration of this ion are poorly studied. In eukaryotic cells, the calcium-ATPase of the SERCA type plays an important role in calcium homeostasis by catalyzing the active efflux of calcium from cytoplasm to endoplasmic reticulum. Here, we reported the identification of SERCA of E. invadens (EiSERCA). This protein contains a putative sequence for endoplasmic reticulum retention and all domains involved in calcium transport identified in mammalian SERCA. By immunofluorescence assays, an antibody against SERCA of E. histolytica detected EiSERCA in a vesicular network in the cytoplasm of E. invadens trophozoites, co-localizing with calreticulin. Interestingly, EiSERCA was redistributed close to plasma membrane during encystation, suggesting that this pump could participate in regulate the calcium concentration during this process. In addition, thapsigargin and cyclopiazonic acid, both specific inhibitors of SERCA, affected the number and structure of cysts, supporting the hypothesis that calcium flux mediated by SERCA has an important role in the life cycle of Entamoeba.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 89, December 2015, Pages 18–26
نویسندگان
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