کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3416713 | 1225134 | 2011 | 5 صفحه PDF | دانلود رایگان |
Variation in disease severity among Escherichia coli O157:H7 infections may result from differential expression of Shiga toxin 2 (Stx2). Eleven strains belonging to four prominent phylogenetic clades, including clade 8 strains representative of the 2006 U.S. spinach outbreak, were examined for stx2 expression by real-time PCR and western blot analysis. Clade 8 strains were shown to overexpress stx2 basally, and following induction with ciprofloxacin when compared to strains from clades 1–3. Differences in stx2 expression generally correlated with Stx2 protein levels. Single-nucleotide polymorphisms identified in regions upstream of stx2AB in clade 8 strains were largely absent in non-clade 8 strains. This study concludes that stx2 overexpression is common to strains from clade 8 associated with hemolytic uremic syndrome, and describes SNPs which may affect stx2 expression and which could be useful in the genetic differentiation of highly-virulent strains.
► Stx2 production by E. coli O157:H7 is associated with severe disease.
► Strains of E. coli O157:H7 from clade 8 overexpress Stx2 compared to other clades.
► SNPs in clade 8 strains may influence Stx2 expression or be useful for detection.
Journal: Microbial Pathogenesis - Volume 51, Issue 6, December 2011, Pages 466–470