کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416876 1225157 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of hemagglutinin/protease expression by the VarS/VarA–CsrA/B/C/D system in Vibrio cholerae
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Regulation of hemagglutinin/protease expression by the VarS/VarA–CsrA/B/C/D system in Vibrio cholerae
چکیده انگلیسی

In this study, through the analysis of Vibrio cholerae 2740-80 mutant strains produced by the cholera toxin subunit B gene containing Mariner-based transposon, we found that disruption of the varS gene, a member of the recently reported sensory system VarS/VarA–CsrA/B/C/D, resulted in altered expression of hemagglutinin/protease A. To further investigate the connection between VarS and HapA, we generated an additional varS mutant, V. cholerae 2740-80-VS, and examined the effect of this mutation on expression of HapA and of genes in the VarS/VarA–CsrA/B/C/D system. 2740-80-VS showed decreased expression of varS, csrB/C, hapR, and hapA along with increased biofilm production. Interestingly, expression of the alternative sigma factor σs, which is important for adaptation to environmental stress, was also decreased in this mutant. These results indicate that the VarS/VarA–CsrA/B/C/D system is involved in the control of HapA expression and biofilm production in V. cholerae 2740-80 through HapR regulation, and also that VarS/VarA controls expression of σs for HapA regulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 48, Issue 6, June 2010, Pages 245–250
نویسندگان
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