کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3416893 1225159 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
EstA protein, a novel virulence factor of Streptococcus pneumoniae, induces nitric oxide and pro-inflammatory cytokine production in RAW 264.7 macrophages through NF-κB/MAPK
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
EstA protein, a novel virulence factor of Streptococcus pneumoniae, induces nitric oxide and pro-inflammatory cytokine production in RAW 264.7 macrophages through NF-κB/MAPK
چکیده انگلیسی

In the present study we characterized the molecular mechanism by which esterase A (EstA) protein, a novel virulence factor of Streptococcus pneumoniae induces inflammation. Stimulation of RAW 264.7 macrophages with purified EstA protein induced the expression of inducible nitrogen oxide synthase (iNOS) mRNA and nitrogen oxide (NO) production in a concentration-dependent manner. Inhibitors of iNOS, NF-κB, p38 and ERK 1/2 MAPK pathways significantly decreased (50–78%) EstA-induced NO production. Similarly, EstA induced TNF-α, IL-1β and IL-6 mRNA expression in RAW 264.7 macrophages in a dose-dependent manner, and pre-treatment of the cell cultures with specific NF-κB, p38 and ERK 1/2 MAPK pathway inhibitors significantly decreased EstA-induced TNF-α, IL-1β and IL-6 protein production. Furthermore, immunoblot analysis revealed the degradation of the inhibitory kappa B (IKB-α) in response to EstA stimulation. Taken together, our data suggests that EstA protein is a novel inducer of NO and pro-inflammatory cytokines by activating the NF-κB, p38 and ERK 1/2 MAPK pathways during inflammatory responses. Future studies on the upstream protein kinases of the MAPK/NF-κB pathways and the kinetics of cytokine production will provide further details into the mechanism of EstA-induced inflammatory response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 47, Issue 4, October 2009, Pages 196–201
نویسندگان
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