کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417248 1593744 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mycoplasma pneumoniae subtype-independent induction of proinflammatory cytokines in THP-1 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Mycoplasma pneumoniae subtype-independent induction of proinflammatory cytokines in THP-1 cells
چکیده انگلیسی

Mycoplasma pneumoniae can be divided into two main subtypes depending on the amino acid sequences of the P1 adhesin and the P65 protein, both located in the attachment organelle. Differences between these subtypes in infectivity, virulence and interaction with host cells have not been extensively studied. Using ELISA to measure released protein and real-time PCR to quantify mRNA, we have demonstrated that both M. pneumoniae subtypes significantly increased tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) at comparable levels in THP-1 cells over a 72 h period of time. However, subtype 2 induced a statistically significant increase (P<0.001) in the release of interleukin-1β at 24 h post-infection compared to subtype 1. These data provide evidence that the induction of proinflammatory cytokine gene and protein expression by M. pneumoniae is not dependent on the infecting subtype.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 40, Issue 6, June 2006, Pages 286–292
نویسندگان
, , , ,