کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417360 1593741 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mycoplasma pneumoniae host–pathogen studies in an air–liquid culture of differentiated human airway epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Mycoplasma pneumoniae host–pathogen studies in an air–liquid culture of differentiated human airway epithelial cells
چکیده انگلیسی

The interaction between Mycoplasma pneumonaie and the airway epithelium in vivo is complex and multifaceted. While multiple in vitro studies have been conducted studying this interaction with cell lines and animal cell and organ culture models, the interactions between M. pneumoniae and fully differentiated human airway epithelium in air–liquid interface culture remains unexplored. In the present study we investigated M. pneumoniae interactions with airway epithelium utilizing an air–liquid interface culture of differentiated normal human bronchial epithelial (NHBE) cells. Utilizing confocal microscopy we found that M. pneumoniae cells bound initially to ciliated epithelial cells, but colonization became more evenly distributed over the entire surface with time. M. pneumoniae infection resulted in stimulation of intercellular adhesion molecule 1 (ICAM-1) gene expression and soluble ICAM-1 production in this culture system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 42, Issues 2–3, February–March 2007, Pages 98–103
نویسندگان
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