کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3417372 | 1593742 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Focal adhesion kinase is involved in type III group B streptococcal invasion of human brain microvascular endothelial cells
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کلمات کلیدی
SDSPAGEkiloDaltonFAKkDaPaxillinGAPDHGFPIgGPBSDMSO - DMSOPI3 kinase - PI3 کینازpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدInvasion - تهاجمDimethylsulfoxide - دیمتیل سولفواکسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتHuman brain microvascular endothelial cell - سلول اندوتلیال مغز استخوان مغز انسانPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریGreen fluorescence protein - پروتئین فلورسانس سبزImmunoglobulin gamma - گاما ایمونوگلوبولینgroup B Streptococcus - گروه B استرپتوکوکglyceraldehydes 3-phosphate dehydrogenase - گلیسرولید هایید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروب شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Focal adhesion kinase is involved in type III group B streptococcal invasion of human brain microvascular endothelial cells Focal adhesion kinase is involved in type III group B streptococcal invasion of human brain microvascular endothelial cells](/preview/png/3417372.png)
چکیده انگلیسی
Group B streptococcus (GBS), the leading cause of neonatal meningitis, has been shown to invade human brain microvascular endothelial cells (HBMEC), which constitute the blood-brain barrier. GBS invasion of HBMEC has been shown to require the host cell actin cytoskeleton rearrangements. The present study examined the mechanisms underlying actin cytoskeleton rearrangements that are involved in type III GBS invasion of HBMEC. We showed that type III GBS invasion was inhibited by genistein, a general tyrosine kinase inhibitor (mean 54% invasion decrease at 100 μM), and LY294002, a phosphatidylinositol 3 (PI3) kinase inhibitor (mean 70% invasion decrease at 50 μM), but not by PP2, an inhibitor of the Src family tyrosine kinases. We subsequently showed that the focal adhesion kinase (FAK) was the one of the host proteins tyrosine phosphorylated by type III GBS. Over-expression of a dominant negative form of the FAK C-terminal domain significantly decreased type III GBS invasion of HBMEC (mean 51% invasion decrease). In addition, we showed that FAK phosphorylation correlated with its association of paxillin, an adapter protein of actin filament, and PI3-kinase subunit p85. This is the first demonstration that FAK phosphorylation and its association with paxillin and PI3 kinase play a key role in type III GBS invasion of HBMEC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbial Pathogenesis - Volume 41, Issues 4â5, OctoberâNovember 2006, Pages 168-173
Journal: Microbial Pathogenesis - Volume 41, Issues 4â5, OctoberâNovember 2006, Pages 168-173
نویسندگان
Sooan Shin, Paul-Satyaseela Maneesh, Jong-Seok Lee, Lewis H. Romer, Kwang Sik Kim,