Effect of feeding strategies on inulinase production analyzed in a biocalorimeter

• Sucrose linear feeding was compared to exponential feeding for inulinase production.
• Maximum inulinase activity of 728 IU/mL was obtained in exponential fed-batch mode.
• The empirical correlation between growth rate and product formation was formulated.
• Metabolic heat release profile correlated well with inulinase production than OUR and CER profiles.

Kluyveromyces marxianus exoinulinase production in shaker and bio reaction calorimeter has been studied. Biocalorimetric studies revealed enhanced inulinase production due to the linear and exponential feeding of sucrose. Linear fed-batch inulinase production (355 IU/mL) was seventeen-fold greater than batch mode (20.98 IU/mL). Further controlling the specific growth rate by exponential feeding of sucrose gave highest inulinase activity of 728 IU/mL. Exponential feeding strategy yielded highest inulinase activity in the synthetic medium with a reduction in batch time to half that of the linear feeding. This has been substantiated by metabolic and physiological aspects of inulinase production. A Michaelis-Menten type empirical model was developed for fed-batch that can be adopted for controlling specific growth rate based on metabolic heat responses, towards achieving maximum enzyme production and release.

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