کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417643 1593772 2015 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of immunodominant proteins of the microalgae Prototheca by proteomic analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروب شناسی
پیش نمایش صفحه اول مقاله
Identification of immunodominant proteins of the microalgae Prototheca by proteomic analysis
چکیده انگلیسی

Prototheca zopfii associated with bovine mastitis and human protothecosis exists as two genotypes, of which genotype 1 is considered as non-infectious and genotype 2 as infectious. The mechanism of infection has not yet been described. The present study was aimed to identify genotype 2-specific immunodominant proteins. Prototheca proteins were separated using two-dimensional gel electrophoresis. Subsequent western blotting with rabbit hyperimmune serum revealed 28 protein spots. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis resulted in the identification of 15 proteins including malate dehydrogenase, elongation factor 1-alpha, heat shock protein 70, and 14-3-3 protein, which were previously described as immunogenic proteins of other eukaryotic pathogens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Microbes and New Infections - Volume 3, January 2015, Pages 37–40
نویسندگان
, , , ,