The upstream sequence segment of the C-terminal cysteine-rich domain is required for microneme trafficking of Plasmodium falciparum erythrocyte binding antigen 175

► Region required for microneme trafficking of P. falciparum EBA-175 was re-assessed. ► Signal sequence and region 6 to cytoplasmic tail are not sufficient. ► There is a sequence segment relatively conserved among EBL members in region 5. ► Deletion or amino acid replacements of this region alters the trafficking. ► A segment in region 5 is also required for correct microneme trafficking of EBA-175.