کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417927 1225484 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Strain variation in the susceptibility and immune response to Clonorchis sinensis infection in mice
چکیده انگلیسی

Mice have shown various susceptibility to infection by Clonorchis sinensis. To compare the intra-specific variation in the host–parasite relationship of C. sinensis, 6 strains of mice (ICR, BALB/c, C57BL/6, DDY, CBA/N, and C3H/HeN) with 3 different haplotypes were evaluated on their susceptibility. The worm recovery rate and immunological responses were observed after 4 and 8 weeks of infection with 30 metacercariae. The highest worm recovery rate was observed as 20.7% in the C3H/HeN strain after 4 weeks of infection along with histopathological changes. The rate was 10.0% in C57BL/6 mice after 8 weeks. ICR, BALB/c, and CBA/N showed elevated levels of IgE at both time points when compared to the rest of the strains. The serum IgG1 and IgG2a levels were elevated in most of the strains; however, the C57BL/6 strain showed a lower level of IgG2a that indicated the IgG1 predominance over IgG2a. The production of IL-4 after concanavalin-A stimulation of splenocytes slightly increased among the mouse strains except C3H/HeN after 4 or 8 weeks of infection, but each strain produced high levels of IFN-γ after 8 weeks, which implied mixed Th1/Th2 responses. ICR, DDY, CBA/N, and C3H/HeN strains showed a significantly increased level of IL-10 after 8 weeks as compared to C57BL/6. All of the strains showed an increased level of IL-13 and suggested fibrotic changes in the mice. In conclusion, mice are insusceptible to infection with C. sinensis; however, the C57BL/6, BALB/c and ICR strains are relatively susceptible after 8 weeks of infection among the six strains. Worm expulsion may be one of the causes of low susceptibility of C3H/HeN mice strain at the 8th week. Elevated IgE, IFN-γ, and IL-13 of infected mice suggest both Th1 and Th2 responses that may be related to the low host susceptibility.

Figure optionsDownload as PowerPoint slideHighlights
► We evaluate six mice strain for Clonorchis sinensis susceptibility.
► We examine histopathological changes in liver, worm recovery and immune response.
► Mice are not susceptible to C. sinensis infection.
► C57BL/6, BALB/c and ICR strain mice are relatively favorable among the strains.
► Elevated IgE, IFN-γ, and IL-13 may be related with the host susceptibility.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 61, Issue 1, March 2012, Pages 118–123
نویسندگان
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