کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3417974 1593781 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of loop-mediated isothermal amplification method for detecting Wuchereria bancrofti DNA in human blood and vector mosquitoes
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Development of loop-mediated isothermal amplification method for detecting Wuchereria bancrofti DNA in human blood and vector mosquitoes
چکیده انگلیسی

We have developed loop-mediated isothermal amplification (LAMP) method to detect Wuchereria bancrofti DNA. The sensitivity and specificity of LAMP method were equivalent to those of PCR method which detects SspI repeat sequence in W. bancrofti genomic DNA: both methods detected one thousandth of W. bancrofti DNA from one microfilaria (Mf), and did not cross-react with DNAs of Brugia malayi, B. pahangi, Dirofilaria immitis, human and Culex quinquefasciatus. We also examined the sensitivity of LAMP using the mimic samples of patient's blood or blood-fed mosquitoes containing one W. bancrofti Mf per sample. The LAMP method was able to detect W. bancrofti DNA in 1000 μl of blood or in a pool of 60 mosquitoes, indicating its usefulness in detecting/monitoring W. bancrofti infection in humans and vector mosquitoes in endemic areas.


► We have developed the LAMP method to detect W. bancrofti DNA.
► The sensitivity and specificity of our LAMP were equivalent to those of PCR which detects SspI repeat sequence.
► This method is superior to PCR in the running cost and the demonstration time.
► This LAMP will be a useful tool for monitoring and evaluating W. bancrofti infection/transmission status in endemic area.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 60, Issue 4, December 2011, Pages 493–497
نویسندگان
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