کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3418156 1225496 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Giardia lamblia behavior during encystment: How morphological changes in shape occur
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Giardia lamblia behavior during encystment: How morphological changes in shape occur
چکیده انگلیسی

Giardia is an intestinal parasite that undergoes adaptation for survival outside the host. Different stages in the Giardia cyst formation include distinctive changes in the trophozoite shape and polarization, from the characteristic flattened dorsal–ventral axis found in motile trophozoites to a rounded appearance and also the appearance of a “tail-like” appendage in later stages of cyst formation. In addition, the flagella disappear and the cyst is oval or rounded and immotile. Since we found no clear information describing how the cells change shape and how the flagella disappear, we applied videomicroscopy, scanning and transmission electron microscopy to follow the gradual modifications that occur in the trophozoite, including alterations in the cell shape, the manner of flagella internalization and changes in disc behavior. Based on the data presented here, it was possible to construct a temporal sequence of changes during Giardia encystation. In this article we show how the membrane growth of the flange contributes to cell shape changes during encystment. In addition, an operculum and flagella internalization is shown. There is a video as a supplement showing these modifications. In other procedure, the plasma membrane was removed and the disc was seen by high resolution scanning electron microscopy where the modifications of the disc spiral can be followed.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Parasitology International - Volume 58, Issue 1, March 2009, Pages 72–80
نویسندگان
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