کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3424161 | 1227198 | 2013 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Membrane association of Wuhan nodavirus protein A is required for its ability to accumulate genomic RNA1 template Membrane association of Wuhan nodavirus protein A is required for its ability to accumulate genomic RNA1 template](/preview/png/3424161.png)
One common feature of positive-strand RNA viruses is the association of viral RNA and viral RNA replicase proteins with specific intracellular membranes to form RNA replication complexes. Wuhan nodavirus (WhNV) encodes protein A, which is the sole viral RNA replicase. Here, we showed that WhNV protein A closely associates with mitochondrial outer membranes and colocalizes with viral RNA replication sites. We further identified the transmembrane domains (N-terminal aa 33-64 and aa 212-254) of protein A for membrane association and mitochondrial localization. Moreover, we found that protein A accumulates genomic RNA by stabilizing the RNA. And our further investigation revealed that the ability of WhNV protein A to associate with membranes is closely linked with its ability for membrane recruitment and stabilization of viral genomic RNA templates. This study represents an advance toward understanding the mechanism of the RNA replication of WhNV and probably other nodaviruses.
► WhNV protein A and it-induced RNA replication localizes to mitochondrial membrane.
► Two specific transmembrane domains are responsible for protein A membrane association.
► Protein A accumulates RNA1 via stabilizing RNA1 and recruiting RNA1 to membranes.
► Membrane association and RNA accumulation of protein A is tightly linked.
Journal: Virology - Volume 439, Issue 2, 10 May 2013, Pages 140–151