کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424196 1227199 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Canine distemper virus with the intact C protein has the potential to replicate in human epithelial cells by using human nectin4 as a receptor
چکیده انگلیسی

Recent outbreaks in monkeys have proven that canine distemper virus (CDV) causes diseases in a wide range of mammals. CDV uses SLAM and nectin4 as receptors to replicate in susceptible animals. Here, we show that human nectin4, but not human SLAM, is fully functional as a CDV receptor. The CDV Ac96I strain hardly replicated in nectin4-expressing human epithelial NCI-H358 cells, but readily adapted to grow in them. Unsurprisingly, no amino acid change in the H protein was required for the adaptation. The original Ac96I strain possessed a truncated C protein, and a subpopulation possessing the intact C protein was selected after growth in NCI-H358 cells. Other CDV strains possessing the intact C protein showed significantly higher growth abilities in NCI-H358 cells than the Ac96I strain with the truncated C protein. These findings suggest that the C protein is functional in human epithelial cells and critical for CDV replication in them.


► Human nectin4 is fully functional as a CDV receptor.
► CDV with a truncated C protein showed growth defect in human epithelial cells.
► Human SLAM is suboptimal as a CDV receptor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 435, Issue 2, 20 January 2013, Pages 485–492
نویسندگان
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