Ultrastructural study of Rift Valley fever virus in the mouse model

Detailed ultrastructural studies of Rift Valley fever virus (RVFV) in the mouse model are needed to develop and characterize a small animal model of RVF for the evaluation of potential vaccines and therapeutics. In this study, the ultrastructural features of RVFV infection in the mouse model were analyzed. The main changes in the liver included the presence of viral particles in hepatocytes and hepatic stem cells accompanied by hepatocyte apoptosis. However, viral particles were observed rarely in the liver; in contrast, particles were extremely abundant in the CNS. Despite extensive lymphocytolysis, direct evidence of viral replication was not observed in the lymphoid tissue. These results correlate with the acute-onset hepatitis and delayed-onset encephalitis that are dominant features of severe human RVF, but suggest that host immune-mediated mechanisms contribute significantly to pathology. The results of this study expand our knowledge of RVFV–host interactions and further characterize the mouse model of RVF.

► Mice were experimentally infected with Rift Valley fever (RVF) virus.
► Electron microscopy was performed on tissues from sequentially sampled mice.
► Viral particles were rare in the liver and abundant in the central nervous system.
► Our findings implicate immune-mediated liver pathology during RVF infection.
► These results support the development of the mouse model of RVF infection.