کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3424335 1594263 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gammaherpesvirus gene expression and DNA synthesis are facilitated by viral protein kinase and histone variant H2AX
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Gammaherpesvirus gene expression and DNA synthesis are facilitated by viral protein kinase and histone variant H2AX
چکیده انگلیسی

Gammaherpesvirus protein kinases are an attractive therapeutic target as they support lytic replication and latency. Via an unknown mechanism these kinases enhance expression of select viral genes and DNA synthesis. Importantly, the kinase phenotypes have not been examined in primary cell types. Mouse gammaherpesvirus-68 (MHV68) protein kinase orf36 activates the DNA damage response (DDR) and facilitates lytic replication in primary macrophages. Significantly, H2AX, a DDR component and putative orf36 substrate, enhances MHV68 replication. Here we report that orf36 facilitated expression of RTA, an immediate early MHV68 gene, and DNA synthesis during de novo infection of primary macrophages. H2AX expression supported efficient RTA transcription and phosphorylated H2AX associated with RTA promoter. Furthermore, viral DNA synthesis was attenuated in H2AX-deficient macrophages, suggesting that the DDR system was exploited throughout the replication cycle. The interactions between a cancer-associated gammaherpesvirus and host tumor suppressor system have important implications for the pathogenesis of gammaherpesvirus infection.


► Gammaherpesvirus protein kinase facilitates immediate early gene expression.
► H2AX, a critical DNA damage response protein, contributes to viral gene expression.
► Gammaherpesvirus protein kinase is required for efficient viral DNA synthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 420, Issue 2, 25 November 2011, Pages 73–81
نویسندگان
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